Modifying Enzymes
Gold Biotechnology Inc DNase I, Bovine Pancreas (Lysate Tested), >300 Kunitz U/mg 1 g
DNase I (Deoxyribonuclease I) is a dialyzed lyophilized powder from bovine pancreas that has been purified by chromatography.
Sigma Aldrich Fine Chemicals Biosciences Catalase from Corynebacterium glutamicum | 9001-05-2 | MFCD00081483 | 5ml
Catalase from Corynebacterium glutamicumPurity: MW: | 9001-05-2 | MFCD00081483 | 5ml
Sigma Aldrich Fine Chemicals Biosciences Catalase from Bovine Liver | 9001-05-2 | MFCD00081483 | 1g
Catalase from Bovine Liver | 9001-05-2 | MFCD00081483 | 1g
AVIDITY LLC BIO-500 Biotin Solution, 5 mM d-biotin, 10 mL, 10 mM Bicine buffer, pH 8.3, Filter sterilized (0.22 μm), Room temperature shipping, -20 °C storage
The AVIDITY LLC BIO500 is a 5mM d-biotin solution supplied in a 10mL volume, designed for critical molecular biology applications. It is particularly suitable for in-vivo biotinylation culture inductions utilizing pBirAcm BirA over-expression plasmids and AviTag™-protein fusion vectors. The solution is also appropriate for large-scale BirA reactions requiring supplemental d-biotin and for supplementing induction growth media. Filter sterilized through a 0.22μm filter, this product ensures sterility for sensitive scientific protocols.
- Concentration: 5 mM d-biotin
- Volume: 10 mL
- Buffer: 10 mM Bicine
- pH: 8.3
- Sterility: Filter Sterilized (0.22 μm)
- Shipping Condition: Room temperature
- Storage Temperature: -20 °C
- Primary Application: In-vivo biotinylation
- Secondary Application: Large scale BirA reactions
LGC GENOMICS LLC (Lucigen) T4 Polynucleotide Kinase, Cloned, 3,000 U @ 10 U/LAn enzyme for the phosphorylation 5' hydroxy ends of ssDNA and dsDNA, RNA, and nucleoside 3 monophosphates.
Phosphorylate 5' hydroxy ends of ssDNA and dsDNA, RNA, and nucleoside 3 monophosphatesHighly active enzyme that readily phosphorylates 5' hydroxyl ends of nucleic acidsFlexible: Use newly phosphorylated nucleic acids in a variety of applications such as cloning, next-gen sequencing library prep, and preparation of labelled nucleic acidsEach T4 Polynucleotide Kinase includes the enzyme and 10X Reaction Buffer without ATP. ATP is available separately.
Worthington Biochemical Corporation DNASEI 20 MG
Deoxyribonuclease I, Chromatographically purified, Equivalent to 1X crystallized Dnase, A lyophilized powder with glycine as a stabilizer, Store at 2 to 8degreeC, Pack Size: 20mg, Source: Bovine Pancreas, min. activity: 2000U/mgKunitz dry w
LGC GENOMICS LLC (Lucigen) Baseline-ZERO DNase, 5000 UDigest large or small DNA molecules better than standard DNase I.
Baseline-ZERO™ DNase digests dsDNA and ssDNA into mononucleotides more effectively than the commonly used bovine pancreatic DNase I.
New England Biolabs, Inc. Exonuclease V (RecBCD) – 1000 units
Exonuclease V, a RecBCD complex from E. coli has several different enzyme activities, including an ATP-dependent single-stranded DNA endonuclease activity, ss- and ds- DNA exonuclease activity. The hydrolysis in each case is bi-directional (from both the 3' and 5' ends) and processive, producing oligonucleotides. All Exonuclease V activities have divalent cation requirements. Mg2+ is required for the exonuclease activity, while Ca2+ inhibits the exonuclease activity and allows double-stranded DNA unwinding (helicase activity) without hydrolysis.
LGC GENOMICS LLC (Lucigen) RNA 5' Polyphosphatase, 200U @ 20 U/LDephosphorylate tri- and di-phosphorylated RNA molecules while avoiding 5'-capped and mono-phosphorylated RNAs.
RNA 5 Polyphosphatase is a Mg2+-independent phosphohydrolase discovered and characterized by Epicentre scientists. The enzyme sequentially removes the and phosphates from 5-triphosphorylated RNA (such as primary RNA transcripts): 5 pppNOH 3 5 pNOH 3 + 2 Pi
Sigma Aldrich Fine Chemicals Biosciences Tyrosinase from mushroom, 9002-10-2, MFCD00082118, 25 KU
Lyophilized powder, ≥1000 unit/mg solid, Synonym: Catechol Oxidase, Monophenol Monooxygenase, Monophenol, dihydroxyphenylalanine:oxygen oxidoreductase, Polyphenol Oxidase. Tyrosinase activity has been assessed in a study that developed an alternative therapeutic agent for treating hyperpigmentation. Tyrosinase has also been used in a study to investigate the oculocutaneous albinism phenotype in the Pakistani population.
Sigma Aldrich Fine Chemicals Biosciences Neuraminidase from Vibrio cholerae, 9001-67-6, MFCD00131711, 1 unit
Type III, buffered aqueous solution, 0.2 μm filtered, 1-5 units/mg protein (Lowry, using NAN-lactose), Synonym: Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase; Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release.
New England Biolabs, Inc. Template Switching RT Enzyme Mix – 20 reactions
The reverse transcriptase (RT) in the Template Switching RT Enzyme Mix adds a few non-templated nucleotides after it reaches the 5' end of the RNA template. These non-templated nucleotides can anneal to a template switching oligo (TSO) with a known sequence, prompting the reverse transcriptase to switch template from RNA to the TSO. The resulting cDNA contains a known sequence (complementary to the sequence of the TSO) attached to the 3' end. This feature can be utilized in a variety of downstream applications, such as cDNA amplification, 5' RACE (rapid amplification of cDNA ends), and 2nd strand cDNA synthesis.
Supply Solutions Roche - DNase I; Grade II, from bovine pancreas; pkg of 100 mg
Roche - DNase I; Grade II, from bovine pancreas; pkg of 100 mg
Sigma Aldrich Fine Chemicals Biosciences DNase I recombinant, RNase-free, 10000 units
DNase I recombinant, RNase-free may be used to degrade DNA in applications that are sensitive to the presence of Rnase.
LGC GENOMICS LLC (Lucigen) Ampligase DNA Ligase without Buffer, 10,000 U @ 5 U/LHighly thermostable DNA ligase for applications where ligation at high temperature is beneficial or required.
Ampligase Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3-hydroxylated and 5-phosphorylated termini in duplex DNA structures that are stable at high temperatures